With the culture of various explant under certain cultural conditions, embryogenesis will result. In this research mbryos were obtained from several species of Hyoscyamus: H.arachnoideus H.pusillus, H.niger, from 2 distinct geographical regions, on MS mediums with various hormonal treatments. The results from statistical assessments showed that the highest embryogenesis were obtained with the following conditions: Culture of region 2 biennial H.niger stern segments on mediums of No .I (IAA=0.2mg/L), No. 3 (BAP=1.5mg/L), No. 4 (Kin=1.5mg/L), No. 7 (BAP=1.5, IAA=0.2mg/L), and No. 10 (Kin= 1.5, IAA=0.2mg/L). Culture of region 2 biennial H. niger roots on mediums of No. 4, 7, 10, and (BAP=1.12, IAA=0.09mg/L). Culture of region 2 biennial H. niger leaves on medium contianing Kin (1.5 mg/L). In addition, the highest embryogenesis frequencies were observed with: Culture of H.pusillus sterns on mediums No. 7; and 12. Culture of H.pusillus leaves on medium No. 3. Culture of H.arachnoideus leaves and sterns on mediums No. 7, and 12. Culture of region I biennial H.niger sterns on mediums No. 7, and 12. Culture of region 2 biennial H.niger roots, sterns, and leaves on medium No. 4, 7, and 12. Culture of region 2 biennial Hniger roots, and sterns on mediums No. 1, 3, and 10. The formation of plantlet resulted from the subculture of the obtained embryos on MS medium and MS medium contianing IAA (0.2mg/L).